Type: HPLC Application #: 108340
Detection: UV, 280nm Conditions: eluent A: 20mM TRIS / acetic acid pH 8.0, eluent B: A) + 1.5M NaCl, gradient from 10 to 45% B in 20min flow rate 1ml/min pressure 150bar
eluent A: 20mM TRIS / acetate pH 8.0, eluent B: A) + 0.75M NaCl, gradient from 0 to 100% B in 10min flow rate 1ml/min pressure 20bar Authors: Macherey-Nagel Source: LC department
Details
Legend: Separation of ovalbumin components in analytical and semipreparative amounts:
A) 20µg, B) 100µg, C) 1000µg, D) 10000µg.
Ovalbumin separation a) before and b) after 10min column treatment with 0.1M